tailieunhanh - báo cáo khoa học: " Structure, expression differentiation and evolution of duplicated fiber developmental genes in Gossypium barbadense and G. hirsutum"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Structure, expression differentiation and evolution of duplicated fiber developmental genes in Gossypium barbadense and G. hirsutum | Zhu et al. BMC Plant Biology 2011 11 40 http 1471-2229 11 40 BMC Plant Biology RESEARCH ARTICLE Open Access Structure expression differentiation and evolution of duplicated fiber developmental genes in Gossypium barbadense and G. hirsutum Huayu Zhu Xiaoyong Han Junhong Lv Liang Zhao Xiaoyang Xu Tianzhen Zhang Wangzhen Guo Abstract Background Both Gossypium hirsutum and G. barbadense probably originated from a common ancestor but they have very different agronomic and fiber quality characters. Here we selected 17 fiber development-related genes to study their structures tree topologies chromosomal location and expression patterns to better understand the interspecific divergence of fiber development genes in the two cultivated tetraploid species. Results The sequence and structure of genes were conserved with the same exon length and numbers in different species while genes showed diversity. There were 15 genes showing independent evolution between the A- and D-subgenomes after polyploid formation while two evolved via different degrees of colonization. Chromosomal location showed that 22 duplicate genes were located in which at least one fiber quality QTL was detected. The molecular evolutionary rates suggested that the D-subgenome of the allotetraploid underwent rapid evolutionary differentiation and selection had acted at the tetraploid level. Expression profiles at fiber initiation and early elongation showed that the transcripts levels of most genes were higher in Hai7124 than in TM-1. During the primary-secondary transition period expression of most genes peaked earlier in TM-1 than in Hai7124. Homeolog expression profile showed that A-subgenome or the combination of A- and D-subgenomes played critical roles in fiber quality divergence of G. hirsutum and G. barbadense. However the expression of D-subgenome alone also played an important role. Conclusion Integrating analysis of the structure and expression to fiber development

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