tailieunhanh - báo cáo khoa học: " Macromolecular composition of phloem exudate from white lupin (Lupinus albus L.)"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Macromolecular composition of phloem exudate from white lupin (Lupinus albus L.) | Rodriguez-Medina et al. BMC Plant Biology 2011 11 36 http 1471-2229 11 36 BMC Plant Biology RESEARCH ARTICLE Open Access Macromolecular composition of phloem exudate from white lupin Lupinus albus L. 2 2 2 3 Caren Rodriguez-Medina Craig A Atkins Anthea J Mann Megan E Jordan Penelope MC Smith Abstract Background Members of the legume genus Lupinus exude phloem spontaneously from incisions made to the vasculature. This feature was exploited to document macromolecules present in exudate of white lupin Lupinus albus L. cv Kiev mutant in particular to identify proteins and RNA molecules including microRNA miRNA . Results Proteomic analysis tentatively identified 86 proteins from 130 spots collected from 2D gels analysed by partial amino acid sequence determination using MS MS. Analysis of a cDNA library constructed from exudate identified 609 unique transcripts. Both proteins and transcripts were classified into functional groups. The largest group of proteins comprised those involved in metabolism 24 followed by protein modification turnover 9 redox regulation 8 cell structural components 6 stress and defence response 6 with fewer in other groups. More prominent proteins were cyclophilin ubiquitin a glycine-rich RNA-binding protein a group of proteins that comprise a glutathione ascorbate-based mechanism to scavenge oxygen radicals enzymes of glycolysis and other metabolism including methionine and ethylene synthesis. Potential signalling macromolecules such as transcripts encoding proteins mediating calcium level and the Flowering locus T FT protein were also identified. From around 330 small RNA clones 18-25 nt 12 were identified as probable miRNAs by homology with those from other species. miRNA composition of exudate varied with site of collection . upward versus downward translocation streams and nutrition . phosphorus level . Conclusions This is the first inventory of macromolecule composition of phloem exudate from a species in