tailieunhanh - báo cáo khoa học: " Study of ‘Redhaven’ peach and its white-fleshed mutant suggests a key role of CCD4 carotenoid dioxygenase in carotenoid and norisoprenoid volatile metabolism"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Study of ‘Redhaven’ peach and its white-fleshed mutant suggests a key role of CCD4 carotenoid dioxygenase in carotenoid and norisoprenoid volatile metabolism | Brandi et al. BMC Plant Biology 2011 11 24 http 1471-2229 11 24 BMC Plant Biology RESEARCH ARTICLE Open Access Study of Redhaven peach and its white-fleshed mutant suggests a key role of CCD4 carotenoid dioxygenase in carotenoid and norisoprenoid volatile metabolism c z D r I I 1 c I D r2 t c I l I z I I r ii 1 - r 3t ỉ I_I z - 4t c k l í I I I rs-r I5 t I t I P- II I I i- 1-eaerica Brandi Einat Bar -abienne iviourgues Gyorgyi Horvatn Erika lurcsi Giovanni Giuliano 1 7 2 3 Alessandro Liverani Stefano Tartarini tfraim Lewinsohn Carlo Rosati Abstract Background Carotenoids are plant metabolites which are not only essential in photosynthesis but also important quality factors in determining the pigmentation and aroma of flowers and fruits. To investigate the regulation of carotenoid metabolism as related to norisoprenoids and other volatile compounds in peach Prunus persica L. Batsch. and the role of carotenoid dioxygenases in determining differences in flesh color phenotype and volatile composition the expression patterns of relevant carotenoid genes and metabolites were studied during fruit development along with volatile compound content. Two contrasted cultivars the yellow-fleshed Redhaven RH and its white-fleshed mutant Redhaven Bianca RHB were examined. Results The two genotypes displayed marked differences in the accumulation of carotenoid pigments in mesocarp tissues. Lower carotenoid levels and higher levels of norisoprenoid volatiles were observed in RHB which might be explained by differential activity of carotenoid cleavage dioxygenase CCD enzymes. In fact the ccd4 transcript levels were dramatically higher at late ripening stages in RHB with respect to RH. The two genotypes also showed differences in the expression patterns of several carotenoid and isoprenoid transcripts compatible with a feed-back regulation of these transcripts. Abamine SG - an inhibitor of CCD enzymes - decreased the levels of both isoprenoid and .