tailieunhanh - báo cáo khoa học: " Identification of rhizome-specific genes by genome-wide differential expression Analysis in Oryza longistaminata"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification of rhizome-specific genes by genome-wide differential expression Analysis in Oryza longistaminata | Hu et al. BMC Plant Biology 2011 11 18 http 1471-2229 11 18 BMC Plant Biology RESEARCH ARTICLE Open Access Identification of rhizome-specific genes by genome-wide differential expression Analysis in Oryza longistaminata f 1f 1 4 1 1 2 -2 Fengyi Hu Di Wang Xiuqin Zhao Ting Zhang Haixi Sun Linghua Zhu Fan Zhang Lijuan Li Qiong Li Dayun Tao2 Binying Fu 1 Zhikang Li1 5 Abstract Background Rhizomatousness is a key component of perenniality of many grasses that contribute to competitiveness and invasiveness of many noxious grass weeds but can potentially be used to develop perennial cereal crops for sustainable farmers in hilly areas of tropical Asia. Oryza longistaminata a perennial wild rice with strong rhizomes has been used as the model species for genetic and molecular dissection of rhizome development and in breeding efforts to transfer rhizome-related traits into annual rice species. In this study an effort was taken to get insights into the genes and molecular mechanisms underlying the rhizomatous trait in O. longistaminata by comparative analysis of the genome-wide tissue-specific gene expression patterns of five different tissues of O. longistaminata using the Affymetrix GeneChip Rice Genome Array. Results A total of 2 566 tissue-specific genes were identified in five different tissues of O. longistaminata including 58 and 61 unique genes that were specifically expressed in the rhizome tips RT and internodes RI respectively. In addition 162 genes were up-regulated and 261 genes were down-regulated in RT compared to the shoot tips. Six distinct cis-regulatory elements CGACG GCCGCC GAGAC AACGG CATGCA and TAAAG were found to be significantly more abundant in the promoter regions of genes differentially expressed in RT than in the promoter regions of genes uniformly expressed in all other tissues. Many of the RT and or RI specifically or differentially expressed genes were located in the QTL regions associated with rhizome expression .