tailieunhanh - báo cáo khoa học: " Identification of drought-response genes and a study of their expression during sucrose accumulation and water deficit in sugarcane culms"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification of drought-response genes and a study of their expression during sucrose accumulation and water deficit in sugarcane culms | Iskandar et al. BMC Plant Biology 2011 11 12 http 1471 -2229 11 12 BMC Plant Biology RESEARCH ARTICLE Open Access Identification of drought-response genes and a study of their expression during sucrose accumulation and water deficit in sugarcane culms I_I Iv I I I- lzZ l r 1 2 4 D fs r E r I 1 1 A I AZ I E IS-. f V3 Cl I r- 1 V Czh m I z l f 3 I I r y- i Z1 VI I 1 5 Hayau M isKandar Rosanne E Casu Andrew I Fletcher Susanne scnmidt Jingsneng xu Donald J Maclean2 John M Manners1 Graham D Bonnett1 Abstract Background The ability of sugarcane to accumulate high concentrations of sucrose in its culm requires adaptation to maintain cellular function under the high solute load. We have investigated the expression of 51 genes implicated in abiotic stress to determine their expression in the context of sucrose accumulation by studying mature and immature culm internodes of a high sucrose accumulating sugarcane cultivar. Using a sub-set of eight genes expression was examined in mature internode tissues of sugarcane cultivars as well as ancestral and more widely related species with a range of sucrose contents. Expression of these genes was also analysed in internode tissue from a high sucrose cultivar undergoing water deficit stress to compare effects of sucrose accumulation and water deficit. Results A sub-set of stress-related genes that are potentially associated with sucrose accumulation in sugarcane culms was identified through correlation analysis and these included genes encoding enzymes involved in amino acid metabolism a sugar transporter and a transcription factor. Subsequent analysis of the expression of these stress-response genes in sugarcane plants that were under water deficit stress revealed a different transcriptional profile to that which correlated with sucrose accumulation. ror example genes with homology to late embryogenesis abundant-related proteins and dehydrin were strongly induced under water deficit but this did not correlate