tailieunhanh - báo cáo khoa học: " Harpin-induced expression and transgenic overexpression of the phloem protein gene AtPP2-A1 in Arabidopsis repress phloem feeding of the green peach aphid Myzus persicae"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Harpin-induced expression and transgenic overexpression of the phloem protein gene AtPP2-A1 in Arabidopsis repress phloem feeding of the green peach aphid Myzus persicae | Zhang et al. BMC Plant Biology 2011 11 11 http 1471-2229 11 11 BMC Plant Biology RESEARCH ARTICLE Open Access Harpin-induced expression and transgenic overexpression of the phloem protein gene AtPP2-A1 in Arabidopsis repress phloem feeding of the green peach aphid Myzus persicae 1Ỷ 1Ỷ 1Ỷ 1 1 1 Chunling Zhang Haojie Shi Lei Chen Xiaomeng Wang Beibei Lu Shuping Zhang Yuan Liang Ruoxue Liu1 Jun Qian1 Weiwei Sun1 Zhenzhen You1 Hansong Dong1 Abstract Background Treatment of plants with HrpNEa a protein of harpin group produced by Gram-negative plant pathogenic bacteria induces plant resistance to insect herbivores including the green peach aphid Myzus persicae a generalist phloem-feeding insect. Under attacks by phloem-feeding insects plants defend themselves using the phloem-based defense mechanism which is supposed to involve the phloem protein 2 PP2 one of the most abundant proteins in the phloem sap. The purpose of this study was to obtain genetic evidence for the function of the Arabidopsis thaliana Arabidopsis PP2-encoding gene AtPP2-A1 in resistance to M. persicae when the plant was treated with HrpNEa and after the plant was transformed with AtPP2-A1. Results The electrical penetration graph technique was used to visualize the phloem-feeding activities of apterous agamic M. persicae females on leaves of Arabidopsis plants treated with HrpNEa and an inactive protein control respectively. A repression of phloem feeding was induced by HrpNEa in wild-type WT Arabidopsis but not in atpp2-a1 E 142 the plant mutant that had a defect in the AtPP2-A1 gene the most HrpNEa-responsive of 30 AtPP2 genes. In WT rather than atpp2-a1 E 142 the deterrent effect of HrpNEa treatment on the phloem-feeding activity accompanied an enhancement of AtPP2-A1 expression. In PP2OETAt AtPP2-A1 -overexpression transgenic Arabidopsis thaliana plants abundant amounts of the AtPP2-A1 gene transcript were detected in different organs including leaves stems calyces and

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