tailieunhanh - báo cáo khoa học: " Field transcriptome revealed critical developmental and physiological transitions involved in the expression of growth potential in japonica rice"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Field transcriptome revealed critical developmental and physiological transitions involved in the expression of growth potential in japonica rice | Sato et al. BMC Plant Biology 2011 11 10 http 1471-2229 11 10 BMC Plant Biology RESEARCH ARTICLE Open Access Field transcriptome revealed critical developmental and physiological transitions involved in the expression of growth potential in japonica rice 1Ỷ 1 Ỷ 2 1 11 Yutaka Sato Baltazar Antonio Nobukazu Namiki Ritsuko Motoyama Kazuhiko Sugimoto Hinako Takehisa Hiroshi Minami2 Kaori Kamatsuki2 Makoto Kusaba3 Hirohiko Hirochika1 Yoshiaki Nagamura1 Abstract Background Plant growth depends on synergistic interactions between internal and external signals and yield potential of crops is a manifestation of how these complex factors interact particularly at critical stages of development. As an initial step towards developing a systems-level understanding of the biological processes underlying the expression of overall agronomic potential in cereal crops a high-resolution transcriptome analysis of rice was conducted throughout life cycle of rice grown under natural field conditions. Results A wide range of gene expression profiles based on 48 organs and tissues at various developmental stages identified 731 organ tissue specific genes as well as 215 growth stage-specific expressed genes universally in leaf blade leaf sheath and root. Continuous transcriptome profiling of leaf from transplanting until harvesting further elucidated the growth-stage specificity of gene expression and uncovered two major drastic changes in the leaf transcriptional program. The first major change occurred before the panicle differentiation accompanied by the expression of RFT1 a putative florigen gene in long day conditions and the downregulation of the precursors of two microRNAs. This transcriptome change was also associated with physiological alterations including phosphate-homeostasis state as evident from the behavior of several key regulators such as miR399. The second major transcriptome change occurred just after flowering and based on analysis of sterile mutant