tailieunhanh - báo cáo khoa học: " Molecular and functional analyses of COPT/Ctrtype copper transporter-like gene family in rice"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Molecular and functional analyses of COPT/Ctrtype copper transporter-like gene family in rice | Yuan et al. BMC Plant Biology 2011 11 69 http 1471-2229 11 69 BMC Plant Biology RESEARCH ARTICLE Open Access Molecular and functional analyses of COPT Ctr-type copper transporter-like gene family in rice Meng Yuan Xianghua Li Jinghua Xiao and Shiping Wang Abstract Background The copper Cu transporter COPT Ctr gene family has an important role in the maintenance of Cu homeostasis in different species. The rice COPT-type gene family consists of seven members COPT1 to COPT7 . However only two COPT1 and COPT5 have been characterized for their functions in Cu transport. Results Here we report the molecular and functional characterization of the other five members of the rice COPT gene family COPT2 COPT3 COPT4 COPT6 and COPT7 . All members of the rice COPT family have the conserved features of known COPT Ctr-type Cu transporter genes. Among the proteins encoded by rice COPTs COPT2 COPT3 and COPT4 physically interacted with COPT6 respectively except for the known interaction between COPT1 and COPT5. COPT2 COPT3 or COPT4 cooperating with COPT6 mediated a high-affinity Cu uptake in the yeast Saccharomyces cerevisiae mutant that lacked the functions of ScCtr1 and ScCtr3 for Cu uptake. COPT7 alone could mediate a high-affinity Cu uptake in the yeast mutant. None of the seven COPTs alone or in cooperation could complement the phenotypes of S. cerevisiae mutants that lacked the transporter genes either for iron uptake or for zinc uptake. However these COPT genes which showed different tissue-specific expression patterns and Cu level-regulated expression patterns were also transcriptionally influenced by deficiency of iron manganese or zinc. Conclusion These results suggest that COPT2 COPT3 and COPT4 may cooperate with COPT6 respectively and COPT7 acts alone for Cu transport in different rice tissues. The endogenous concentrations of iron manganese or zinc may influence Cu homeostasis by influencing the expression of COPTs in rice. Background Copper Cu is .