tailieunhanh - báo cáo khoa học: " HC-Pro silencing suppressor significantly alters the gene expression profile in tobacco leaves and flowers"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: HC-Pro silencing suppressor significantly alters the gene expression profile in tobacco leaves and flowers | Soitamo et al. BMC Plant Biology 2011 11 68 http 1471-2229 11 68 BMC Plant Biology RESEARCH ARTICLE Open Access HC-Pro silencing suppressor significantly alters the gene expression profile in tobacco leaves and flowers Arto J Soitamo Balaji Jada and Kirsi Lehto Abstract Background RNA silencing is used in plants as a major defence mechanism against invasive nucleic acids such as viruses. Accordingly plant viruses have evolved to produce counter defensive RNA-silencing suppressors RSSs . These factors interfere in various ways with the RNA silencing machinery in cells and thereby disturb the microRNA miRNA mediated endogene regulation and induce developmental and morphological changes in plants. In this study we have explored these effects using previously characterized transgenic tobacco plants which constitutively express under CaMV 35S promoter the helper component-proteinase HC-Pro derived from a potyviral genome. The transcript levels of leaves and flowers of these plants were analysed using microarray techniques Tobacco 4 X 44 k Agilent . Results Over expression of HC-Pro RSS induced clear phenotypic changes both in growth rate and in leaf and flower morphology of the tobacco plants. The expression of 748 and 332 genes was significantly changed in the leaves and flowers respectively in the HC-Pro expressing transgenic plants. Interestingly these transcriptome alterations in the HC-Pro expressing tobacco plants were similar as those previously detected in plants infected with ssRNA-viruses. Particularly many defense-related and hormone-responsive genes . ethylene responsive transcription factor 1 ERF1 were differentially regulated in these plants. Also the expression of several stress-related genes and genes related to cell wall modifications protein processing transcriptional regulation and photosynthesis were strongly altered. Moreover genes regulating circadian cycle and flowering time were significantly altered which may have .