tailieunhanh - báo cáo khoa học: " Reactive oxygen species and transcript analysis upon excess light treatment in wild-type Arabidopsis thaliana vs a photosensitive mutant lacking zeaxanthin and lutein"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Reactive oxygen species and transcript analysis upon excess light treatment in wild-type Arabidopsis thaliana vs a photosensitive mutant lacking zeaxanthin and lutein | Alboresi et al. BMC Plant Biology 2011 11 62 http 1471-2229 11 62 BMC Plant Biology RESEARCH ARTICLE Open Access Reactive oxygen species and transcript analysis upon excess light treatment in wild-type Arabidopsis thaliana vs a photosensitive mutant lacking zeaxanthin and lutein A laccunrlr i A IHc iroci 1 t I I Pi3 IKOcfm1 t A loccim A r li ilo2 DotrnnD r KĨIIr i3 PkìkD Dr iK iK Ki li 4 I I lirti c f f i olli2 K lM Alessandro Alboresi Luca Dall Osto Alessio Aprile PctlUllld CallllU Limca Roncaglia Luigi Cattivelli and Roberto Bassi1 Abstract Background Reactive oxygen species ROS are unavoidable by-products of oxygenic photosynthesis causing progressive oxidative damage and ultimately cell death. Despite their destructive activity they are also signalling molecules priming the acclimatory response to stress stimuli. Results To investigate this role further we exposed wild type Arabidopsis thaliana plants and the double mutant npq1lut2 to excess light. The mutant does not produce the xanthophylls lutein and zeaxanthin whose key roles include ROS scavenging and prevention of ROS synthesis. Biochemical analysis revealed that singlet oxygen 1O2 accumulated to higher levels in the mutant while other ROS were unaffected allowing to define the transcriptomic signature of the acclimatory response mediated by 1O2 which is enhanced by the lack of these xanthophylls species. The group of genes differentially regulated in npq1lut2 is enriched in sequences encoding chloroplast proteins involved in cell protection against the damaging effect of ROS. Among the early fine-tuned components are proteins involved in tetrapyrrole biosynthesis chlorophyll catabolism protein import folding and turnover synthesis and membrane insertion of photosynthetic subunits. Up to now the flu mutant was the only biological system adopted to define the regulation of gene expression by 1O2. In this work we propose the use of mutants accumulating 1O2 by mechanisms different from