tailieunhanh - báo cáo khoa học: " Identification of differentially expressed genes induced by Bamboo mosaic virus infection in Nicotiana benthamiana by cDNA-amplified fragment length polymorphism"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification of differentially expressed genes induced by Bamboo mosaic virus infection in Nicotiana benthamiana by cDNA-amplified fragment length polymorphism | Cheng et al. BMC Plant Biology 2010 10 286 http 1471-2229 10 286 BMC Plant Biology RESEARCH ARTICLE Open Access Identification of differentially expressed genes induced by Bamboo mosaic virus infection in Nicotiana benthamiana by cDNA-amplified fragment length polymorphism 1 11 1 Shun-Fang Cheng Ying-Ping Huang Zi-Rong Wu Chung-Chi Hu Yau-Heiu Hsu Ching-Hsiu Tsai Abstract Background The genes of plants can be up- or down-regulated during viral infection to influence the replication of viruses. Identification of these differentially expressed genes could shed light on the defense systems employed by plants and the mechanisms involved in the adaption of viruses to plant cells. Differential gene expression in Nicotiana benthamiana plants in response to infection with Bamboo mosaic virus BaMV was revealed using cDNA-amplified fragment length polymorphism AFLP . Results Following inoculation with BaMV N. benthamiana displayed differential gene expression in response to the infection. Isolation cloning and sequencing analysis using cDNA-AFLP furnished 90 cDNA fragments with eight pairs of selective primers. Fifteen randomly selected genes were used for a combined virus-induced gene silencing VIGS knockdown experiment using BaMV infection to investigate the roles played by these genes during viral infection specifically addressing the means by which these genes influence the accumulation of BaMV protein. Nine of the 15 genes showed either a positive or a negative influence on the accumulation of BaMV protein. Six knockdown plants showed an increase in the accumulation of BaMV suggesting that they played a role in the resistance to viral infection while three plants showed a reduction in coat protein indicating a positive influence on the accumulation of BaMV in plants. An interesting observation was that eight of the nine plants showing an increase in BaMV coat protein were associated with cell rescue defense death aging signal transduction .