tailieunhanh - báo cáo khoa học: " Molecular, genetic and transcriptional evidence for a role of VvAGL11 in stenospermocarpic seedlessness in grapevine"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Molecular, genetic and transcriptional evidence for a role of VvAGL11 in stenospermocarpic seedlessness in grapevine | BMC Plant Biology Molecular genetic and transcriptional evidence for a role of VvAGL11 in stenospermocarpic seedlessness in grapevine Mejia et al. BioMed Central Mejía et al. BMC Plant Biology 2011 11 57 http 1471-2229 11 57 29 March 2011 Mejia et al. BMC Plant Biology 2011 11 57 http 1471-2229 11 57 BMC Plant Biology RESEARCH ARTICLE Open Access Molecular genetic and transcriptional evidence for a role of VvAGL11 in stenospermocarpic seedlessness in grapevine 1 1 1 1 2 1 Nilo Mejia Braulio Soto Marcos Guerrero Ximena Casanueva Cléa Houel María de los Ángeles Miccono Rodrigo Ramos1 Loic Le Cunff3 Jean-Michel Boursiquot3 Patricio Hinrichsen1 and Anne-Frangoise Adam-Blondon2 Abstract Background Stenospermocarpy is a mechanism through which certain genotypes of Vitis vinifera L. such as Sultanina produce berries with seeds reduced in size. Stenospermocarpy has not yet been characterized at the molecular level. Results Genetic and physical maps were integrated with the public genomic sequence of Vitis vinifera L. to improve QTL analysis for seedlessness and berry size in experimental progeny derived from a cross of two seedless genotypes. Major QTLs co-positioning for both traits on chromosome 18 defined a 92-kb confidence interval. Functional information from model species including Vitis suggested that VvAGLll included in this confidence interval might be the main positional candidate gene responsible for seed and berry development. Characterization of VvAGL11 at the sequence level in the experimental progeny identified several SNPs and INDELs in both regulatory and coding regions. In association analyses performed over three seasons these SNPs and INDELs explained up to 78 and 44 of the phenotypic variation in seed and berry weight respectively. Moreover genetic experiments indicated that the regulatory region has a larger effect on the phenotype than the coding region. Transcriptional analysis lent additional support to .