tailieunhanh - báo cáo khoa học: " Glutathione synthesis is essential for pollen germination in vitro"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Glutathione synthesis is essential for pollen germination in vitro | Zechmann et al. BMC Plant Biology 2011 11 54 http 1471-2229 11 54 BMC Plant Biology RESEARCH ARTICLE Open Access Glutathione synthesis is essential for pollen germination in vitro Bernd Zechmann1 2 Barbara E Koffler1 and Scott D Russell3 Abstract Background The antioxidant glutathione fulfills many important roles during plant development growth and defense in the sporophyte however the role of this important molecule in the gametophyte generation is largely unclear. Bioinformatic data indicate that critical control enzymes are negligibly transcribed in pollen and sperm cells. Therefore we decided to investigate the role of glutathione synthesis for pollen germination in vitro in Arabidopsis thaliana accession Col-0 and in the glutathione deficient mutant pad2-1 and link it with glutathione status on the subcellular level. Results The depletion of glutathione by buthionine sulfoximine BSO an inhibitor of glutathione synthesis reduced pollen germination rates to 2-5 compared to 71 germination in wildtype controls. The application of reduced glutathione GSH together with BSO restored pollen germination and glutathione contents to control values demonstrating that inhibition of glutathione synthesis is responsible for the decrease of pollen germination in vitro. The addition of indole-3-acetic acid IAA to media containing BSO restored pollen germination to control values which demonstrated that glutathione depletion in pollen grains triggered disturbances in auxin metabolism which led to inhibition of pollen germination. Conclusions This study demonstrates that glutathione synthesis is essential for pollen germination in vitro and that glutathione depletion and auxin metabolism are linked in pollen germination and early elongation of the pollen tube as IAA addition rescues glutathione deficient pollen. Keywords Arabidopsis auxin gametophyte glutathione indole-3-acetic acid pollen Background Glutathione is an important antioxidant and redox buffer