tailieunhanh - báo cáo khoa học: " Differential miRNA expression in Rehmannia glutinosa plants subjected to continuous cropping"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Differential miRNA expression in Rehmannia glutinosa plants subjected to continuous cropping | Yang et al. BMC Plant Biology 2011 11 53 http 1471-2229 11 53 BMC Plant Biology RESEARCH ARTICLE Open Access Differential miRNA expression in Rehmannia glutinosa plants subjected to continuous cropping Yanhui Yangt Xinjian Chen1 Junying Chen1 Haixia Xu1 Juan Lit and Zhongyi Zhang Abstract Background The productivity of the medicinally significant perennial herb Rehmannia glutinosa is severely affected after the first year of cropping. While there is some information available describing the physiological and environmental causes of this yield decline there is as yet no data regarding the changes in gene expression which occur when the species is continuously cropped. Results Using a massively parallel Solexa DNA sequencing platform it was possible to identify and quantify the abundance of a large number of R. glutinosa miRNAs. We contrasted the miRNA content of first year crop plants with that of second year crop ones and were able to show that of 89 conserved belonging to 25 families and six novel miRNAs six families 29 of the former and three of the latter were differentially expressed. The three novel miRNAs were predicted to target seven genes and the 29 conserved ones 308 genes. The potential targets of 32 of these differentially expressed miRNAs involved in the main transcription regulation plant development and signal transduction. A functional analysis of the differentially expressed miRNAs suggested that several of the proposed targets could be directly or indirectly responsible for the development of the tuberous root. Conclusion We have compared differential miRNAs expression in the first year crop FP R. glutinosa plants and second year crop SP ones. The outcome identifies some potential leads for understanding the molecular basis of the processes underlying the difficulty of maintaining the productivity of continuously cropped R. glutinosa. Background Rehmannia glutinosa L. is a perennial herbaceous species belonging to the .