tailieunhanh - báo cáo khoa học: "A microarray approach to identify genes involved in seed-pericarp cross-talk and development in peach"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A microarray approach to identify genes involved in seed-pericarp cross-talk and development in peach | Bonghi et al. BMC Plant Biology 2011 11 107 http 1471 -2229 11 107 BMC Plant Biology RESEARCH ARTICLE Open Access A microarray approach to identify genes involved in seed-pericarp cross-talk and development in peach 1Ỷ 2Ỷ 12 1 1 Claudio Bonghi Livio Trainotti Alessandro Botton Alice Tadiello Angela Rasori Fiorenza Ziliotto Valerio Zaffalon1 Giorgio Casadoro2 and Angelo Ramina1 Abstract Background Field observations and a few physiological studies have demonstrated that peach embryogenesis and fruit development are tightly coupled. In fact attempts to stimulate parthenocarpic fruit development by means of external tools have failed. Moreover physiological disturbances during early embryo development lead to seed abortion and fruitlet abscission. Later in embryo development the interactions between seed and fruit development become less strict. As there is limited genetic and molecular information about seed-pericarp crosstalk and development in peach a massive gene approach based on the use of the gPEACH array platform and quantitative real time RT-PCR qRT-PCR was used to study this process. Results A comparative analysis of the transcription profiles conducted in seed and mesocarp cv Fantasia throughout different developmental stages S1 S2 S3 and S4 evidenced that 455 genes are differentially expressed in seed and fruit. Among differentially expressed genes some were validated as markers in two subsequent years and in three different genotypes. Seed markers were a LTP1 lipid transfer protein a PR pathogenesis-related protein a prunin and LEA Late Embryogenesis Abundant protein for S1 S2 S3 and S4 respectively. Mesocarp markers were a RD22-like protein a serin-carboxypeptidase a senescence related protein and an Aux IAA for S1 S2 S3 and S4 respectively. The microarray data analyzed by using the HORMONOMETER platform allowed the identification of hormoneresponsive genes some of them putatively involved in seed-pericarp crosstalk. Results .