tailieunhanh - báo cáo khoa học: " Meiosis-specific gene discovery in plants: RNA-Seq applied to isolated Arabidopsis male meiocytes"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Meiosis-specific gene discovery in plants: RNA-Seq applied to isolated Arabidopsis male meiocytes | BMC Plant Biology Meiosis-specific gene discovery in plants RNA-Seq applied to isolated Arabidopsis male meiocytes Chen et a . Chen et al. BMC Plant Biology 2010 10 280 http 1471-2229 10 280 17 December 2010 2 BioMed Central Chen et al. BMC Plant Biology 2010 10 280 http 1471-2229 10 280 BMC Plant Biology RESEARCH ARTICLE Open Access Meiosis-specific gene discovery in plants RNA-Seq applied to isolated Arabidopsis male meiocytes 1 2 2 4 2 2 2 Changbin Chen Andrew D Farmer Raymond J Langley Joann Mudge John A Crow Gregory D May James Huntley2 3 Alan G Smith1 Ernest F Retzel2 Abstract Background Meiosis is a critical process in the reproduction and life cycle of flowering plants in which homologous chromosomes pair synapse recombine and segregate. Understanding meiosis will not only advance our knowledge of the mechanisms of genetic recombination but also has substantial applications in crop improvement. Despite the tremendous progress in the past decade in other model organisms . Saccharomyces cerevisiae and Drosophila melanogaster the global identification of meiotic genes in flowering plants has remained a challenge due to the lack of efficient methods to collect pure meiocytes for analyzing the temporal and spatial gene expression patterns during meiosis and for the sensitive identification and quantitation of novel genes. Results A high-throughput approach to identify meiosis-specific genes by combining isolated meiocytes RNA-Seq bioinformatic and statistical analysis pipelines was developed. By analyzing the studied genes that have a meiosis function a pipeline for identifying meiosis-specific genes has been defined. More than 1 000 genes that are specifically or preferentially expressed in meiocytes have been identified as candidate meiosis-specific genes. A group of 55 genes that have mitochondrial genome origins and a significant number of transposable element TE genes 1 036 were also found to have up-regulated