tailieunhanh - báo cáo khoa học: " Exploiting EST databases for the development and characterization of EST-SSR markers in castor bean (Ricinus communis L.)"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Exploiting EST databases for the development and characterization of EST-SSR markers in castor bean (Ricinus communis L.) | Qiu et al. BMC Plant Biology 2010 10 278 http 1471-2229 10 278 BMC Plant Biology RESEARCH ARTICLE Open Access Exploiting EST databases for the development and characterization of EST-SSR markers in castor bean Ricinus communis L. Lijun Qiu1 3 Chun Yang1 Bo Tian1 Jun-Bo Yang2 Aizhong Liu1 Abstract Background The castor bean Ricinus communis L a monotypic species in the spurge family Euphorbiaceae 2n 20 is an important non-edible oilseed crop widely cultivated in tropical sub-tropical and temperate countries for its high economic value. Because of the high level of ricinoleic acid over 85 in its seed oil the castor bean seed derivatives are often used in aviation oil lubricants nylon dyes inks soaps adhesive and biodiesel. Due to lack of efficient molecular markers little is known about the population genetic diversity and the genetic relationships among castor bean germplasm. Efficient and robust molecular markers are increasingly needed for breeding and improving varieties in castor bean. The advent of modern genomics has produced large amounts of publicly available DNA sequence data. In particular expressed sequence tags ESTs provide valuable resources to develop gene-associated SSR markers. Results In total 18 928 publicly available non-redundant castor bean EST sequences representing approximately Mb were evaluated and 7732 SSR sites in 5 122 ESTs were identified by data mining. Castor bean exhibited considerably high frequency of EST-SSRs. We developed and characterized 118 polymorphic EST-SSR markers from 379 primer pairs flanking repeats by screening 24 castor bean samples collected from different countries. A total of 350 alleles were identified from 118 polymorphic SSR loci ranging from 2-6 per locus A with an average of . The EST-SSR markers developed displayed moderate gene diversity He with an average of . Genetic relationships among 24 germplasms were investigated using the genotypes of 350 alleles showing geographic

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