tailieunhanh - báo cáo khoa học: " Isolation and functional characterization of CE1 binding proteins"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Isolation and functional characterization of CE1 binding proteins | Lee et al. BMC Plant Biology 2010 10 277 http 1471-2229 10 277 BMC Plant Biology RESEARCH ARTICLE Open Access Isolation and functional characterization of CE1 binding proteins Sun-ji Lee Ji Hye Park Mi Hun Lee Ji-hyun Yu Soo Young Kim Abstract Background Abscisic acid ABA is a plant hormone that controls seed germination protective responses to various abiotic stresses and seed maturation. The ABA-dependent processes entail changes in gene expression. Numerous genes are regulated by ABA and promoter analyses of the genes revealed that cis-elements sharing the ACGTGGC consensus sequence are ubiquitous among ABA-regulated gene promoters. The importance of the core sequence which is generally known as ABA response element ABRE has been demonstrated by various experiments and its cognate transcription factors known as ABFs AREBs have been identified. Although necessary ABRE alone is not sufficient and another cis-element known as coupling element CE is required for full range ABA-regulation of gene expression. Several CEs are known. However despite their importance the cognate transcription factors mediating ABA response via CEs have not been reported to date. Here we report the isolation of transcription factors that bind one of the coupling elements CE1. Results To isolate CE1 binding proteins we carried out yeast one-hybrid screens. Reporter genes containing a trimer of the CE1 element were prepared and introduced into a yeast strain. The yeast was transformed with library DNA that represents RNA isolated from ABA-treated Arabidopsis seedlings. From the screen of million yeast transformants we isolated 78 positive clones. Analysis of the clones revealed that a group of AP2 ERF domain proteins binds the CE1 element. We investigated their expression patterns and analyzed their overexpression lines to investigate the in vivo functions of the CE element binding factors CEBFs . Here we show that one of the CEBFs AtERF13 confers ABA .

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