tailieunhanh - báo cáo khoa học: " Multiple evidence for the role of an Ovate-like gene in determining fruit shape in pepper"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Multiple evidence for the role of an Ovate-like gene in determining fruit shape in pepper | Tsaballa et al. BMC Plant Biology 2011 11 46 http 1471-2229 11 46 BMC Plant Biology RESEARCH ARTICLE Open Access Multiple evidence for the role of an Ovate-like gene in determining fruit shape in pepper 1 2 2 Aphrodite Tsaballa Konstantinos Pasentsis Nikos Darzentas Athanasios S Tsaftaris Abstract Background Grafting is a widely used technique contributing to sustainable and ecological production of many vegetables but important fruit quality characters such as taste aroma texture and shape are known for years to be affected by grafting in important vegetables species including pepper. From all the characters affected fruit shape is the most easily observed and measured. From research in tomato fruit shape is known to be controlled by many QTLs but only few of them have larger effect on fruit shape variance. In this study we used pepper cultivars with different fruit shape to study the role of a pepper Ovate-like gene CaOvate which encodes a negative regulator protein that brings significant changes in tomato fruit shape. Results We successfully cloned and characterized Ovate-like genes designated as CaOvate from two pepper cultivars of different fruit shape cv. Mytilini Round and cv. Piperaki Long hereafter referred to as cv. Round and cv. Long after the shape of their mature fruits. The CaOvate consensus contains a 1008-bp ORF encodes a 335 amino-acid polypeptide shares 63 identity with the tomato OVATE protein and exhibits high similarity with OVATE sequences from other Solanaceae species all placed in the same protein subfamily as outlined by expert sequence analysis. No significant structural differences were detected between the CaOvate genes obtained from the two cultivars. However relative quantitative expression analysis showed that the expression of CaOvate followed a different developmental profile between the two cultivars being higher in cv. Round . Furthermore down-regulation of CaOvate through VIGS in cv. Round changes its .