tailieunhanh - báo cáo khoa học: " Relative expression of genes of terpene metabolism in different tissues of Artemisia annua L"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Relative expression of genes of terpene metabolism in different tissues of Artemisia annua L | Olofsson et al. BMC Plant Biology 2011 11 45 http 1471-2229 11 45 BMC Plant Biology RESEARCH ARTICLE Open Access Relative expression of genes of terpene metabolism in different tissues of Artemisia annua L Linda Olofsson Alexander Engstrom Anneli Lundgren Peter E Brodelius Abstract Background Recently Artemisia annua L. annual or sweet wormwood has received increasing attention due to the fact that the plant produces the sesquiterpenoid endoperoxide artemisinin which today is widely used for treatment of malaria. The plant produces relatively small amounts of artemisinin and a worldwide shortage of the drug has led to intense research in order to increase the yield of artemisinin. In order to improve our understanding of terpene metabolism in the plant and to evaluate the competition for precursors which may influence the yield of artemisinin we have used qPCR to estimate the expression of 14 genes of terpene metabolism in different tissues. Results The four genes of the artemisinin biosynthetic pathway amorpha-4 11-diene synthase amorphadiene-12-hydroxylase artemisinic aldehyde A11 13 reductase and aldehyde dehydrogenase 1 showed remarkably higher expression between 40- to 500-fold in flower buds and young leaves compared to other tissues old leaves stems roots hairy root cultures . Further dihydroartemisinic aldehyde reductase showed a very high expression only in hairy root cultures. Germacrene A and caryophyllene synthase were mostly expressed in young leaves and flower buds while epi-cedrol synthase was highly expressed in old leaves. 3-Hydroxy-3-methyl-glutaryl coenzyme A reductase exhibited lower expression in old leaves compared to other tissues. Farnesyldiphosphate synthase squalene synthase and 1-deoxy-D-xylulose-5-phosphate reductoisomerase showed only modest variation in expression in the different tissues while expression of 1-deoxy-D-xylulose-5-phosphate synthase was 7-8-fold higher in flower buds and young leaves compared to old