tailieunhanh - báo cáo khoa học: " Characterisation of the legume SERK-NIK gene superfamily including splice variants: Implications for development and defence"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Characterisation of the legume SERK-NIK gene superfamily including splice variants: Implications for development and defence | Nolan et al. BMC Plant Biology 2011 11 44 http 1471-2229 11 44 BMC Plant Biology RESEARCH ARTICLE Open Access Characterisation of the legume SERK-NIK gene superfamily including splice variants Implications for development and defence Kim E Nolan Sergey Kurdyukov Ray J Rose Abstract Background SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE SERK genes are part of the regulation of diverse signalling events in plants. Current evidence shows SERK proteins function both in developmental and defence signalling pathways which occur in response to both peptide and steroid ligands. SERKs are generally present as small gene families in plants with five SERK genes in Arabidopsis. Knowledge gained primarily through work on Arabidopsis SERKs indicates that these proteins probably interact with a wide range of other receptor kinases and form a fundamental part of many essential signalling pathways. The SERK1 gene of the model legume Medicago truncatula functions in somatic and zygotic embryogenesis and during many phases of plant development including nodule and lateral root formation. However other SERK genes in M. truncatula and other legumes are largely unidentified and their functions unknown. Results To aid the understanding of signalling pathways in M. truncatula we have identified and annotated the SERK genes in this species. Using degenerate PCR and database mining eight more SERK-like genes have been identified and these have been shown to be expressed. The amplification and sequencing of several different PCR products from one of these genes is consistent with the presence of splice variants. Four of the eight additional genes identified are upregulated in cultured leaf tissue grown on embryogenic medium. The sequence information obtained from M. truncatula was used to identify SERK family genes in the recently sequenced soybean Glycine max genome. Conclusions A total of nine SERK or SERK-like genes have been identified in M. truncatula and .