tailieunhanh - báo cáo khoa học: "Gene family structure, expression and functional analysis of HD-Zip III genes in angiosperm and gymnosperm forest trees"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Gene family structure, expression and functional analysis of HD-Zip III genes in angiosperm and gymnosperm forest trees | Côté et al. BMC Plant Biology 2010 10 273 http 1471-2229 10 273 BMC Plant Biology RESEARCH ARTICLE Open Access Gene family structure expression and functional analysis of HD-Zip III genes in angiosperm and gymnosperm forest trees Z-I 1 1 3 2 2 Caroline L Côté Francis Boileau Vicky Roy Mario Ouellet Caroline Levasseur Marie-Josée Morency Janice EK Cooke4 Armand Séguin2 John J MacKay 1 Abstract Background Class III Homeodomain Leucine Zipper HD-Zip III proteins have been implicated in the regulation of cambium identity as well as primary and secondary vascular differentiation and patterning in herbaceous plants. They have been proposed to regulate wood formation but relatively little evidence is available to validate such a role. We characterised and compared HD-Zip III gene family in an angiosperm tree Populus spp. poplar and the gymnosperm Picea glauca white spruce representing two highly evolutionarily divergent groups. Results Full-length cDNA sequences were isolated from poplar and white spruce. Phylogenetic reconstruction indicated that some of the gymnosperm sequences were derived from lineages that diverged earlier than angiosperm sequences and seem to have been lost in angiosperm lineages. Transcript accumulation profiles were assessed by RT-qPCR on tissue panels from both species and in poplar trees in response to an inhibitor of polar auxin transport. The overall transcript profiles HD-Zip III complexes in white spruce and poplar exhibited substantial differences reflecting their evolutionary history. Furthermore two poplar sequences homologous to HD-Zip III genes involved in xylem development in Arabidopsis and Zinnia were over-expressed in poplar plants. PtaHBl overexpression produced noticeable effects on petiole and primary shoot fibre development suggesting that PtaHBl is involved in primary xylem development. We also obtained evidence indicating that expression of PtaHBl affected the transcriptome by altering the .