tailieunhanh - báo cáo khoa học: "Structure and expression of the maize (Zea mays L.) SUN-domain protein gene family: evidence for the existence of two divergent classes of SUN proteins in plants"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Structure and expression of the maize (Zea mays L.) SUN-domain protein gene family: evidence for the existence of two divergent classes of SUN proteins in plants | Structure and expression of the maize Zea mays L. SUNdomain protein gene family evidence for the existence of two divergent classes of SUN proteins in plants Murphy et al. Murphy et al. BMC Plant Biology 2010 10 269 http 1471-2229 10 269 8 December 2010 BioMed Central Murphy et al. BMC Plant Biology 2010 10 269 http 1471-2229 10 269 BMC Plant Biology RESEARCH ARTICLE Open Access Structure and expression of the maize Zea mays L. SUN-domain protein gene family evidence for the existence of two divergent classes of SUN proteins in plants Shaun P Murphy1 Carl R Simmons2 Hank W Bass1 3 Abstract Background The nuclear envelope that separates the contents of the nucleus from the cytoplasm provides a surface for chromatin attachment and organization of the cortical nucleoplasm. Proteins associated with it have been well characterized in many eukaryotes but not in plants. SUN Sad1p Unc-84 domain proteins reside in the inner nuclear membrane and function with other proteins to form a physical link between the nucleoskeleton and the cytoskeleton. These bridges transfer forces across the nuclear envelope and are increasingly recognized to play roles in nuclear positioning nuclear migration cell cycle-dependent breakdown and reformation of the nuclear envelope telomere-led nuclear reorganization during meiosis and karyogamy. Results We found and characterized a family of maize SUN-domain proteins starting with a screen of maize genomic sequence data. We characterized five different maize ZmSUN genes ZmSUN1-5 which fell into two classes probably of ancient origin as they are also found in other monocots eudicots and even mosses . The first ZmSUNl 2 here designated canonical C-terminal SUN-domain CCSD includes structural homologs of the animal and fungal SUN-domain protein genes. The second ZmSUN3 4 5 here designated plant-prevalent mid-SUN 3 transmembrane PM3 includes a novel but conserved structural variant SUN-domain protein gene .