tailieunhanh - báo cáo khoa học: " Differential expression of cysteine desulfurases in soybean"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Differential expression of cysteine desulfurases in soybean | Heis et al. BMC Plant Biology 2011 11 166 http 1471-2229 11 166 BMC Plant Biology RESEARCH ARTICLE Open Access Differential expression of cysteine desulfurases in soybean 1 1 1 2 1 Marta D Heis Elisabeth M Ditmer Luisa A de Oliveira Ana Paula G Frazzon Rogério Margis and Jeverson Frazzon3 Abstract Background Iron-sulfur Fe-S clusters are prosthetic groups required to sustain fundamental life processes including electron transfer metabolic reactions sensing signaling gene regulation and stabilization of protein structures. In plants the biogenesis of Fe-S protein is compartmentalized and adapted to specific needs of the cell. Many environmental factors affect plant development and limit productivity and geographical distribution. The impact of these limiting factors is particularly relevant for major crops such as soybean which has worldwide economic importance. Results Here we analyze the transcriptional profile of the soybean cysteine desulfurases NFS1 NFS2 and ISD11 genes involved in the biogenesis of Fe-S clusters by quantitative RT-PCR. NFS1 ISD11 and NFS2 encoding two mitochondrial and one plastid located proteins respectively are duplicated and showed distinct transcript levels considering tissue and stress response. NFS1 and ISD11 are highly expressed in roots whereas NFS2 showed no differential expression in tissues. Cold-treated plants showed a decrease in NFS2 and ISD11 transcript levels in roots and an increased expression of NFS1 and ISD11 genes in leaves. Plants treated with salicylic acid exhibited increased NFS1 transcript levels in roots but lower levels in leaves. In silico analysis of promoter regions indicated the presence of different cis-elements in cysteine desulfurase genes in good agreement with differential expression of each locus. Our data also showed that increasing of transcript levels of mitochondrial genes NFS1 ISD11 are associated with higher activities of aldehyde oxidase and xanthine dehydrogenase two .