tailieunhanh - báo cáo khoa học: " Gene expression profile analysis of tobacco leaf trichomes"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Gene expression profile analysis of tobacco leaf trichomes | Cui et al. BMC Plant Biology 2011 11 76 http 1471-2229 11 76 BMC Plant Biology RESEARCH ARTICLE Open Access Gene expression profile analysis of tobacco leaf trichomes Hong Cui1 Song-Tao Zhang1 Hui-Juan Yang1 Hao Ji1 and Xiu-Jie Wang2 Abstract Background Leaf trichomes of Nicotiana tabacum are distinguished by their large size high density and superior secretion ability. They contribute to plant defense response against biotic and abiotic stress and also influence leaf aroma and smoke flavor. However there is limited genomic information about trichomes of this non-model plant species. Results We have characterized Nicotiana tabacum leaf trichome gene expression using two approaches. In the first a trichome cDNA library was randomly sequenced and 2831 unique genes were obtained. The most highly abundant transcript was ribulose bisphosphate carboxylase RuBisCO . Among the related sequences most encoded enzymes involved in primary metabolism. Secondary metabolism related genes such as isoprenoid and flavonoid biosynthesis-related were also identified. In the second approach a cDNA microarray prepared from these 2831 clones was used to compare gene expression levels in trichome and leaf. There were 438 differentially expressed genes between trichome and leaves-minus-trichomes. Of these 207 highly expressed genes in tobacco trichomes were enriched in second metabolic processes defense responses and the metabolism regulation categories. The expression of selected unigenes was confirmed by semi-quantitative RT-PCR analysis some of which were specifically expressed in trichomes. Conclusion The expression feature of leaf trichomes in Nicotiana tabacum indicates their metabolic activity and potential importance in stress resistance. Sequences predominantly expressed in trichomes will facilitate genemining and metabolism control of plant trichome. Background Many terrestrial plants are covered with uni- or multicellular epidermal appendages called .