tailieunhanh - báo cáo khoa học: " A pair of orthologs of a leucine-rich repeat receptor kinase-like disease resistance gene family regulates rice response to raised temperature"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A pair of orthologs of a leucine-rich repeat receptor kinase-like disease resistance gene family regulates rice response to raised temperature | Zhang et al. BMC Plant Biology 2011 11 160 http 1471-2229 11 160 BMC Plant Biology RESEARCH ARTICLE Open Access A pair of orthologs of a leucine-rich repeat receptor kinase-like disease resistance gene family regulates rice response to raised temperature Haitao Zhang Yinglong Cao Jing Zhao Xianghua Li Jinghua Xiao and Shiping Wang Abstract Background Rice Xa3 Xa26 disease-resistance gene encodes a leucine-rich repeat LRR receptor kinase-type protein against Xanthomonas oryzae pv. oryzae Xoo and belongs to a multigene family. However the functions of most genes in this family are unknown. Results Here we report that two orthologs of this family the NRKe from rice variety Nipponbare and 9RKe from variety 93-11 at the RKe locus have similar functions although they encode different proteins. This pair of orthologs could not mediate resistance to Xoo but they were transcriptionally induced by raised temperature. Transcriptional activation of NRKe or 9RKe resulted in the formation of temperature-sensitive lesion mimics which were spots of dead cells associated with accumulation of superoxides in different organs of the transgenic plants. These plants were more sensitive to high temperature shock than wild-type controls. Transgenic plants carrying a chimeric protein consisting of the LRR domain of NRKe and the kinase domain of Xa3 Xa26 developed the same lesion mimics as the NRKe-transgenic plants whereas transgenic plants carrying another chimeric protein consisting of the LRR domain of Xa3 Xa26 and the kinase domain of NRKe were free of lesion mimic. All the transgenic plants carrying a chimeric protein were susceptible to Xoo. Conclusion These results suggest that the RKe locus is involved in rice response to raised temperature. The LRR domain of RKe protein appears to be important to sense increased temperature. The RKe-involved temperature-related pathway and Xa3 Xa26-mediated disease-resistance pathway may partially overlap. Background Disease