tailieunhanh - báo cáo khoa học: "Loss-of-function mutations affecting a specific Glycine max R2R3 MYB transcription factor result in brown hilum and brown seed coats"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Loss-of-function mutations affecting a specific Glycine max R2R3 MYB transcription factor result in brown hilum and brown seed coats | Gillman et al. BMC Plant Biology 2011 11 155 http 1471-2229 11 155 BMC Plant Biology RESEARCH ARTICLE Open Access Loss-of-function mutations affecting a specific Glycine max R2R3 MYB transcription factor result in brown hilum and brown seed coats Jason D Gillman1 Ashley Tetlow2 Jeong-Deong Lee3 J Grover Shannon4 and Kristin Bilyeu1 Abstract Background Although modern soybean cultivars feature yellow seed coats with the only color variation found at the hila the ancestral condition is black seed coats. Both seed coat and hila coloration are due to the presence of phenylpropanoid pathway derivatives principally anthocyanins. The genetics of soybean seed coat and hilum coloration were first investigated during the resurgence of genetics during the 1920s following the rediscovery of Mendel s work. Despite the inclusion of this phenotypic marker into the extensive genetic maps developed for soybean over the last twenty years the genetic basis behind the phenomenon of brown seed coats the R locus has remained undetermined until now. Results In order to identify the gene responsible for the r gene effect brown hilum or seed coat color we utilized bulk segregant analysis and identified recombinant lines derived from a population segregating for two phenotypically distinct alleles of the R locus. Fine mapping was accelerated through use of a novel bioinformatically determined set of Simple Sequence Repeat SSR markers which allowed us to delimit the genomic region containing the r gene to less than 200 kbp despite the use of a mapping population of only 100 F6 lines. Candidate gene analysis identified a loss of function mutation affecting a seed coat-specific expressed R2R3 MYB transcription factor gene Glyma09g36990 as a strong candidate for the brown hilum phenotype. We observed a near perfect correlation between the mRNA expression levels of the functional R gene candidate and an UDP-glucose flavonoid 3-O-glucosyltransferase UF3GT gene which is .

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