tailieunhanh - Báo cáo y học: "An N-terminally truncated envelope protein encoded by a human"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: An N-terminally truncated envelope protein encoded by a human . | Roebke et al. Retrovirology 2010 7 69 http content 7 1 69 RETROVIROLOGY RESEARCH Open Access An N-terminally truncated envelope protein encoded by a human endogenous retrovirus W locus on chromosome 1t 1t 1 2 1 1 Christina Roebke Silke Wahl Georg Laufer Christine Stadelmann Marlies Sauter Nikolaus Mueller-Lantzsch Jens Mayer3 Klemens Ruprecht1 4 Abstract Background We previously showed that the envelope env sequence of a human endogenous retrovirus HERV -W locus on chromosome is transcribed in human peripheral blood mononuclear cells. The env open reading frame ORF of this locus is interrupted by a premature stop at codon 39 but otherwise harbors a long ORF for an N-terminally truncated 475 amino acid Env protein starting at an in-frame ATG at codon 68. We set out to characterize the protein encoded by that ORF. Results Transient expression of the 475 amino acid HERV-W env ORF produced an N-terminally truncated HERV-W Env protein as detected by the monoclonal anti-HERV-W Env antibodies 6A2B2 and 13H5A5. Remarkably reversion of the stop at codon 39 in HERV-W env reconstituted a full-length HERV-W Env protein. Similar to the full-length HERV-W Env protein Syncytin-1 reconstituted full-length HERV-W Env is glycosylated forms oligomers and is expressed at the cell surface. In contrast HERV-W Env is unglycosylated does not form oligomers and is located intracellularly probably due to lack of a signal peptide. Finally we reconfirm by immunohistochemistry that monoclonal antibody 6A2B2 detects an antigen expressed in placenta and multiple sclerosis brain lesions. Conclusions A partially defective HERV-W env gene located on chromosome which we propose to designate ERVWE2 has retained coding capacity and can produce ex vivo an N-terminally truncated Env protein named N-Trenv. Detection of an antigen by 6A2B2 in placenta and multiple sclerosis lesions opens the possibility that N-Trenv could be .

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