tailieunhanh - Báo cáo y học: "Absence of evidence of Xenotropic Murine Leukemia Virus-related virus infection in persons with Chronic Fatigue Syndrome and healthy controls in the United States"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Absence of evidence of Xenotropic Murine Leukemia Virus-related virus infection in persons with Chronic Fatigue Syndrome and healthy controls in the United States. | Switzer et al. Retrovirology 2010 7 57 http content 7 1 57 gtr RETR0VIR0L0GY RESEARCH Open Access Absence of evidence of Xenotropic Murine Leukemia Virus-related virus infection in persons with Chronic Fatigue Syndrome and healthy controls in the United States William M Switzer 1 Hongwei Jia1 Oliver Hohn2 HaoQiang Zheng1 Shaohua Tang1 Anupama Shankar1 Norbert Bannert2 Graham Simmons3 R Michael Hendry1 Virginia R Falkenberg4 William C Reeves4 and Walid Heneine1 Abstract Background XMRV a xenotropic murine leukemia virus MuLV -related virus was recently identified by PCR testing in 67 of persons with chronic fatigue syndrome CFS and in of healthy persons from the United States. To investigate the association of XMRV with CFS we tested blood specimens from 51 persons with CFS and 56 healthy persons from the US for evidence of XMRV infection by using serologic and molecular assays. Blinded PCR and serologic testing were performed at the US Centers for Disease Control and Prevention CDC and at two additional laboratories. Results Archived blood specimens were tested from persons with CFS defined by the 1994 international research case definition and matched healthy controls from Wichita Kansas and metropolitan urban and rural Georgia populations. Serologic testing at CDC utilized a Western blot WB assay that showed excellent sensitivity to MuLV and XMRV polyclonal or monoclonal antibodies and no reactivity on sera from 121 US blood donors or 26 HTLV-and HIV-infected sera. Plasma from 51 CFS cases and plasma from 53 controls were all WB negative. Additional blinded screening of the 51 cases and 53 controls at the Robert Koch Institute using an ELISA employing recombinant Gag and Env XMRV proteins identified weak seroreactivity in one CFS case and a healthy control which was not confirmed by immunofluorescence. PCR testing at CDC employed a gag and a pol nested PCR assay with a detection threshold of 10 copies in 1 ug of human DNA. DNA specimens