tailieunhanh - Báo cáo y học: " Use of ultraviolet-light irradiated multiple myeloma cells as immunogens to generate tumor-specific cytolytic T lymphocytes"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Use of ultraviolet-light irradiated multiple myeloma cells as immunogens to generate tumor-specific cytolytic T lymphocytes. | Journal of Immune Based Therapies and Vaccines BioMed Central Original research Use of ultraviolet-light irradiated multiple myeloma cells as immunogens to generate tumor-specific cytolytic T lymphocytes Charles A Gullo 1 2 William YK Hwang2 3 Chye K Poh2 Melvin Au2 Geraline Cow1 2 and Gerrard Teoh2 3 Open Access Address 1Cancer Immunology Laboratory Department of Clinical Research Singapore General Hospital Outram Road 169608 Singapore 2Multiple Myeloma Research Laboratory SingHealth Research Facilities 7 Hospital Drive Block A 02-01 169611 Singapore and 3Department of Hematology Singapore General Hospital SGH Outram Road 169608 Singapore Email Charles A Gullo - William YK Hwang - ghehyk@ Chye K Poh - chykoon@ Melvin Au - melvinau78@ Geraline Cow - geralinexu@ Gerrard Teoh - ghk_teoh@ Corresponding author Published 28 April 2008 Received 6 November 2007 Journal of Immune Based Therapies and Vaccines 2008 6 2 doi l 476-8518-6-2 Accepted 28 April 2008 This article is available from http content 6 1 2 2008 Gullo et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background As the eradication of tumor cells in vivo is most efficiently performed by cytolytic T lymphocytes CTL various methods for priming tumor-reactive lymphocytes have been developed. In this study a method of priming CTLs with ultraviolet UV -irradiated tumor cells which results in termination of tumor cell proliferation apoptosis as well as upregulation of heat shock proteins HSP expression is described. Methods Peripheral blood mononuclear cells PBMC were primed .