tailieunhanh - Báo cáo y học: " Increased APOBEC3G and APOBEC3F expression is associated with low viral load and prolonged survival in simian immunodeficiency virus infected rhesus monkeys"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Increased APOBEC3G and APOBEC3F expression is associated with low viral load and prolonged survival in simian immunodeficiency virus infected rhesus monkeys. | MuBil et al. Retrovirology 2011 8 77 http content 8 1 77 RETR0VIR0L0GY RESEARCH Open Access Increased APOBEC3G and APOBEC3F expression is associated with low viral load and prolonged survival in simian immunodeficiency virus infected rhesus monkeys 22 2 13 Bianka MuBil Ulrike Sauermann Dirk Motzkus Christiane Stahl-Hennig and Sieghart Sopper Abstract Background The cytidine deaminases APOBEC3G A3G and APOBEC3F A3F are innate cellular factors that inhibit replication of a number of viruses including HIV-1. Since antiviral activity of APOBEC3 has been mainly confirmed by in vitro data we examined their role for disease progression in the SIV macaque model for AIDS. Results We quantified A3G and A3F mRNA in PBMC and leukocyte subsets of uninfected and SlVmac-infected rhesus macaques. Compared with uninfected animals we found increased A3G and A3F mRNA levels in PBMC purified CD4 T-cells and CD14 monocytes as well as lymph node cells from asymptomatic SIV-infected macaques. APOBEC3 mRNA levels correlated negatively with plasma viral load and highest amounts of APOBEC3 mRNA were detected in long term non-progressors LTNPs . During acute viremia A3G mRNA increased in parallel with MxA a prototype interferon-stimulated gene indicating a common regulation by the initial interferon response. This association disappeared during the asymptomatic stage. Conclusion Our findings suggest a protective effect of APOBEC3 for HIV and SIV in vivo and indicate regulation of APOBEC3 by interferon during early infection and by contribution of other hitherto undefined factors at later disease stages. Elucidating the regulatory mechanisms leading to increased APOBEC3 mRNA levels in LTNPs could help to develop new therapies against HIV. Background Infection with HIV leads to the development of severe immunodeficiency in a widely variable time frame. A small percentage of the HIV-infected individuals the long term non-progressors LTNPs even remain clinically healthy

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