tailieunhanh - Báo cáo y học: "SIV antigen immunization induces transient antigen-specific T cell responses and selectively activates viral replication in draining lymph nodes in retroviral suppressed rhesus macaques"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: SIV antigen immunization induces transient antigen-specific T cell responses and selectively activates viral replication in draining lymph nodes in retroviral suppressed rhesus macaques. | Hu et al. Retrovirology 2011 8 57 http content 8 1 57 RETR0VIR0L0GY RESEARCH Open Access SIV antigen immunization induces transient antigen-specific T cell responses and selectively activates viral replication in draining lymph nodes in retroviral suppressed rhesus macaques 12 3 2 4 3 Haitao Hu Lucio Gama Pyone P Aye Janice E Clements Peter A Barry Andrew A Lackner and Drew Weissman 1 Abstract Background HIV infection causes a qualitative and quantitative loss of CD4 T cell immunity. The institution of anti-retroviral therapy ART restores CD4 T cell responses to many pathogens but HIV-specific responses remain deficient. Similarly therapeutic immunization with HIV antigens of chronically infected ART treated subjects results in poor induction of HIV-specific CD4 responses. In this study we used a macaque model of ART treatment during chronic infection to study the virologic consequences of SIV antigen stimulation in lymph nodes early after immunization. Rhesus CMV RhCMV seropositive Mamu A 01 positive rhesus macaques were chronically infected with SIVmac251 and treated with ART. The immune and viral responses to SIV gag and RhCMV pp65 antigen immunization in draining lymph nodes and peripheral blood were analyzed. Animals were immunized on contralateral sides with SIV gag and RhCMV pp65 encoding plasmids which allowed lymph nodes draining each antigen to be obtained at the same time from the same animal for direct comparison. Results We observed that both SIV and RhCMV immunizations stimulated transient antigen-specific T cell responses in draining lymph nodes. The RhCMV-specific responses were potent and sustained 50 days postimmunization in the periphery while the SIV-specific responses were transient and extinguished quickly. The SIV antigen stimulation selectively induced transient SIV replication in draining lymph nodes. Conclusions The data are consistent with a model whereby viral replication in response to SIV antigen stimulation .

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