tailieunhanh - Báo cáo y học: "Diagnostic value of real-time polymerase chain reaction to detect viruses in young children admitted to the paediatric intensive care unit with lower respiratory tract infection"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care cung cấp cho các bạn kiến thức về ngành y đề tài: Diagnostic value of real-time polymerase chain reaction to detect viruses in young children admitted to the paediatric intensive care unit with lower respiratory tract infection. | Available online http content 10 2 R61 Research Diagnostic value of real-time polymerase chain reaction to detect viruses in young children admitted to the paediatric intensive care unit with lower respiratory tract infection Alma C van de Pol1 Tom FW Wolfs1 Nicolaas JG Jansen2 Anton M van Loon3 and John WA Rossen3 Open Access Department of Pediatrics Division of Infectious Diseases Wilhelmina Children s Hospital University Medical Center Utrecht Utrecht The Netherlands 2Department of Pediatrics Division of Intensive Care Wilhelmina Children s Hospital University Medical Center Utrecht Utrecht The Netherlands 3Department of Virology Eijkman-Winkler Institute University Medical Center Utrecht Utrecht The Netherlands Corresponding author John WA Rossen Received 8 Dec 2005 Revisions requested 18 Jan 2006 Revisions received 7 Feb 2006 Accepted 17 Mar 2006 Published 12 Apr 2006 Critical Care 2006 10 R61 doi cc4895 This article is online at http content 10 2 R61 2006 van de Pol et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Introduction The aetiology of lower respiratory tract infections in young children admitted to the paediatric intensive care unit PICU is often difficult to establish. However most infections are believed to be caused by respiratory viruses. A diagnostic study was performed to compare conventional viral tests with the recently developed real-time PCR technique. Method Samples from children aged under 5 years presenting to a tertiary PICU suspected of having a lower respiratory tract infection were tested using conventional methods viral culture and immunofluorescence and real-time PCR during the winter season from December 2004 to May