tailieunhanh - Báo cáo y học: "Rapid construction of a dendritic cell vaccine through physical perturbation and apoptotic malignant T cell loading"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Rapid construction of a dendritic cell vaccine through physical perturbation and apoptotic malignant T cell loading. | Journal of Immune Based Therapies and Vaccines Original research BioMed Central Open Access Rapid construction of a dendritic cell vaccine through physical perturbation and apoptotic malignant T cell loading Maria Salskov-Iversen1 Carole L Berger 2 and Richard L Edelson2 Address Department of Immunology AArhus University Aarhus Denmark and 2Department of Dermatology Yale University School of Medicine New Haven CT USA Email Maria Salskov-Iversen - maria@ Carole L Berger - Richard L Edelson - Redeloson@ Corresponding author Published 19 July 2005 Received 04 April 2005 Journal of Immune Based Therapies and Vaccines 2005 3 4 doi 1476- Accepted 19 July 2005 8518-3-4 This article is available from http content 3 1 4 2005 Salskov-Iversen et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We have demonstrated that adherence and release of monocytes from a plastic surface drives their differentiation into immature dendritic cells DC that can mature further during overnight incubation in the presence of apoptotic malignant T cells. Based on these results we sought to develop a clinically practical rapid means for producing DC loaded with malignant cells. A leukapheresis harvest containing the clonal leukemic expansion of malignant CD4 T cells was obtained from the blood of patients with cutaneous T cell lymphoma CTCL . CTCL cells were purified with a CD3-magnetic bead column where CD3 engagement rendered the malignant T cells apoptotic. The monocyte fraction was simultaneously activated by column passage re-added to the apoptotic CTCL cells and co-cultured overnight. CTCL cell apoptosis DC differentiation and apoptotic malignant T cell ingestion