tailieunhanh - Báo cáo y học: "Tat RNA silencing suppressor activity contributes to perturbation of lymphocyte miRNA by HIV-1"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Tat RNA silencing suppressor activity contributes to perturbation of lymphocyte miRNA by HIV-1. | Hayes et al. Retrovirology 2011 8 36 http content 8 1 36 RETR0VIR0L0GY RESEARCH Open Access Tat RNA silencing suppressor activity contributes to perturbation of lymphocyte miRNA by HIV-1 Amy M Hayes1 Shuiming Qian1 2 Lianbo Yu3 and Kathleen Boris-Lawrie1 2 Abstract Background MicroRNA miRNA -mediated RNA silencing is integral to virtually every cellular process including cell cycle progression and response to virus infection. The interplay between RNA silencing and HIV-1 is multifaceted and accumulating evidence posits a strike-counterstrike interface that alters the cellular environment to favor virus replication. For instance miRNA-mediated RNA silencing of HIV-1 translation is antagonized by HIV-1 Tat RNA silencing suppressor activity. The activity of HIV-1 accessory proteins Vpr Vif delays cell cycle progression which is a process prominently modulated by miRNA. The expression profile of cellular miRNA is altered by HIV-1 infection in both cultured cells and clinical samples. The open question stands of what if any is the contribution of Tat RNA silencing suppressor activity or Vpr Vif activity to the perturbation of cellular miRNA by HIV-1. Results Herein we compared the perturbation of miRNA expression profiles of lymphocytes infected with HIV-1NL4-3 or derivative strains that are deficient in Tat RNA silencing suppressor activity Tat K51A substitution or ablated of the vpr vif open reading frames. Microarrays recapitulated the perturbation of the cellular miRNA profile by HIV-1 infection. The miRNA expression trends overlapped 50 with published microarray results on clinical samples from HIV-1 infected patients. Moreover the number of miRNA perturbed by HIV-1 was largely similar despite ablation of Tat RSS activity and Vpr Vif however the Tat RSS mutation lessened HIV-1 downregulation of twenty-two miRNAs. Conclusions Our study identified miRNA expression changes attributable to Tat RSS activity in HIV-1NL4-3. The results accomplish a .