tailieunhanh - Báo cáo y học: "No evidence of XMRV in prostate cancer cohorts in the Midwestern United States"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: No evidence of XMRV in prostate cancer cohorts in the Midwestern United States. | Sakuma et al. Retrovirology 2011 8 23 http content 8 1 23 RETR0VIR0L0GY RESEARCH Open Access No evidence of XMRV in prostate cancer cohorts in the Midwestern United States 1 2 1 1 1 1 Toshie Sakuma Stéphane Hué Karen A Squillace Jason M Tonne Patrick R Blackburn Seiga Ohmine Tayaramma Thatava1 Greg J Towers2 and Yasuhiro Ikeda1 Abstract Background Xenotropic murine leukemia virus MLV -related virus XMRV was initially identified in prostate cancer PCa tissue particularly in the prostatic stromal fibroblasts of patients homozygous for the RNASEL R462Q mutation. A subsequent study reported XMRV antigens in malignant prostatic epithelium and association of XMRV infection with PCa especially higher-grade tumors independently of the RNASEL polymorphism. Further studies showed high prevalence of XMRV or related MLV sequences in chronic fatigue syndrome patients CFS while others found no or low prevalence of XMRV in a variety of diseases including PCa or CFS. Thus the etiological link between XMRV and human disease remains elusive. To address the association between XMRV infection and PCa we have tested prostate tissues and human sera for the presence of viral DNA viral antigens and anti-XMRV antibodies. Results Real-time PCR analysis of 110 PCa Gleason scores 4 and 40 benign and normal prostate tissues identified six positive samples 5 PCa and 1 non-PCa . No statistical link was observed between the presence of proviral DNA and PCa PCa grades and the RNASEL R462Q mutation. The amplified viral sequences were distantly related to XMRV but nearly identical to endogenous MLV sequences in mice. The PCR positive samples were also positive for mouse mitochondrial DNA by nested PCR suggesting contamination of the samples with mouse DNA. Immuno-histochemistry IHC with an anti-XMRV antibody but not an anti-MLV antibody that recognizes XMRV sporadically identified antigen-positive cells in prostatic epithelium irrespectively of the status of viral DNA .

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