tailieunhanh - Báo cáo y học: " No association of xenotropic murine leukemia virus-related virus with prostate cancer or chronic fatigue syndrome in Japa"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: No association of xenotropic murine leukemia virus-related virus with prostate cancer or chronic fatigue syndrome in Japan. | Furuta et al. Retrovirology 2011 8 20 http content 8 1 20 RETR0VIR0L0GY RESEARCH Open Access No association of xenotropic murine leukemia virus-related virus with prostate cancer or chronic fatigue syndrome in Japan Rika A Furuta1 Takayuki Miyazawa2 Takeki Sugiyama3 Hirohiko Kuratsune4 Yasuhiro Ikeda5 Eiji Sato2 6 7 5 9 1 8 1 Naoko Misawa Yasuhito Nakatomi Ryuta Sakuma Kazuta Yasui Kouzi Yamaguti Fumiya Hirayama Abstract Background The involvement of xenotropic murine leukemia virus-related virus XMRV in prostate cancer PC and chronic fatigue syndrome CFS is disputed as its reported prevalence ranges from 0 to 25 in PC cases and from 0 to more than 80 in CFS cases. To evaluate the risk of XMRV infection during blood transfusion in Japan we screened three populations-healthy donors n 500 patients with PC n 67 and patients with CFS n 100 -for antibodies against XMRV proteins in freshly collected blood samples. We also examined blood samples of viral antibody-positive patients with PC and all both antibody-positive and antibody-negative patients with CFS for XMRV DNA. Results Antibody screening by immunoblot analysis showed that a fraction of the cases possessed antiGag antibodies regardless of their gender or disease condition. Most of these antibodies were highly specific to XMRV Gag capsid protein but none of the individuals in the three tested populations retained strong antibody responses to multiple XMRV proteins. In the viral antibody-positive PC patients we occasionally detected XMRV genes in plasma and peripheral blood mononuclear cells but failed to isolate an infectious or full-length XMRV. Further all CFS patients tested negative for XMRV DNA in peripheral blood mononuclear cells. Conclusion Our data show no solid evidence of XMRV infection in any of the three populations tested implying that there is no association between the onset of PC or CFS and XMRV infection in Japan. However the lack of adequate human specimens as a .