tailieunhanh - Báo cáo y học: " Live cell visualization of the interactions between HIV-1 Gag and the cellular RNA-binding protein Staufen1"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Retrovirology cung cấp cho các bạn kiến thức về ngành y đề tài: Live cell visualization of the interactions between HIV-1 Gag and the cellular RNA-binding protein Staufen1. | Milev et al. Retrovirology 2010 7 41 http content 7 1 41 gtr RETR0VIR0L0GY RESEARCH Open Access Live cell visualization of the interactions between HIV-1 Gag and the cellular RNA-binding protein Staufen1 Miroslav P Mi lev 1 2 Chris M Brown3 and Andrew J Mouland 1 2 4 Abstract Background Human immunodeficiency virus type 1 HIV-1 uses cellular proteins and machinery to ensure transmission to uninfected cells. Although the host proteins involved in the transport of viral components toward the plasma membrane have been investigated the dynamics of this process remain incompletely described. Previously we showed that the double-stranded ds RNA-binding protein Staufenl is found in the HIV-1 ribonucleoprotein RNP that contains the HIV-1 genomic RNA vRNA Gag and other host RNA-binding proteins in HIV-1-producing cells. Staufen1 interacts with the nucleocapsid domain NC domain of Gag and regulates Gag multimerization on membranes thereby modulating HIV-1 assembly. The formation of the HIV-1 RNP is dynamic and likely central to the fate of the vRNA during the late phase of the HIV-1 replication cycle. Results Detailed molecular imaging of both the intracellular trafficking of virus components and of virus-host protein complexes is critical to enhance our understanding of factors that contribute to HIV-1 pathogenesis. In this work we visualized the interactions between Gag and host proteins using bimolecular and trimolecular fluorescence complementation BiFC and TriFC analyses. These methods allow for the direct visualization of the localization of protein-protein and protein-protein-RNA interactions in live cells. We identified where the virus-host interactions between Gag and Staufen1 and Gag and IMP1 also known as VICKZ1 IGF2BP1 and ZBP1 occur in cells. These virushost interactions were not only detected in the cytoplasm but were also found at cholesterol-enriched GM1-containing lipid raft plasma membrane domains. Importantly Gag specifically .