tailieunhanh - Báo cáo y học: "Analysis of XMRV integration sites from human prostate cancer tissues suggests PCR contamination rather than genuine human infection"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Analysis of XMRV integration sites from human prostate cancer tissues suggests PCR contamination rather than genuine human infection. | Garson et al. Retrovirology 2011 8 13 http content 8 1 13 RETROVIROLOGY CORRESPONDENCE Open Access Analysis of XMRV integration sites from human prostate cancer tissues suggests PCR contamination rather than genuine human infection Jeremy A Garson1 Paul Kellam1 2 Greg J Towers1 Abstract XMRV is a gammaretrovirus associated in some studies with human prostate cancer and chronic fatigue syndrome. Central to the hypothesis of XMRV as a human pathogen is the description of integration sites in DNA from prostate tumour tissues. Here we demonstrate that 2 of 14 patient-derived sites are identical to sites cloned in the same laboratory from experimentally infected DU145 cells. Identical integration sites have never previously been described in any retrovirus infection. We propose that the patient-derived sites are the result of PCR contamination. This observation further undermines the notion that XMRV is a genuine human pathogen. Introduction XMRV was originally described in 2006 in the tumour tissue of patients with a familial form of prostate cancer associated with mutations that impair the function of the antiviral defence protein RNase L 1 . Three independent groups subsequently reported the presence of XMRV in a significant proportion of prostate cancers but the linkage to polymorphisms of the RNase L gene was not confirmed. In contrast at least seven other studies have reported an inability to detect or extremely low prevalence of XMRV in prostate cancer despite using highly sensitive PCR-based assays. Immunohistological in situ-hybridisation and serological studies have also been inconsistent in their findings. Some studies 1 2 using immunostaining and or FISH detected XMRV in a small percentage of stromal cells but not in tumour cells whereas others using similar techniques reported XMRV predominantly in tumour cells rather than stromal cells. In a recent study Aloia and colleagues 3 employed HPLC purified proteins to raise the antisera used