tailieunhanh - Báo cáo y học: " Contamination of clinical specimens with MLVencoding nucleic acids: implications for XMRV and other candidate human retroviruses"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Contamination of clinical specimens with MLVencoding nucleic acids: implications for XMRV and other candidate human retroviruses. | Smith Retrovirology 2010 7 112 http content 7 1 112 RETR0VIR0L0GY VIEWPOINTS Open Access Contamination of clinical specimens with MLV-encoding nucleic acids implications for XMRV and other candidate human retroviruses Robert A Smith Abstract Efforts to assess the prevalence of xenotropic murine leukemia virus-related virus XMRV in patients with prostate cancer and chronic fatigue syndrome have relied heavily on PCR-based testing of clinical samples and have yielded widely divergent findings. This week in Retrovirology reports from four independent research groups illustrate the extreme care needed to exclude DNA or RNA contamination in PCR analyses of XMRV. In addition phylogenetic evidence suggesting that previously-published XMRV sequences originated from a commonly-used prostate carcinoma cell line 22Rv1 is presented. These findings raise important questions regarding the provenance of XMRV and its potential connection to human disease. Introduction Reports of a newly-discovered gammaretrovirus xeno-tropic murine leukemia virus-related virus XMRV in patients diagnosed with prostate cancer 1 2 and chronic fatigue syndrome CFS 3 have attracted the attention of investigators throughout the retroviral research community. XMRV was initially identified in prostate tumor samples from individuals harboring a specific polymorphism in RNASEL a gene important for interferon-mediated antiviral defense 1 . Studies describing the receptor usage and integration site preference of the virus were soon followed by a second report of XMRV infection in an unrelated c ohort of prostate cancer patients 2 . Although an association between XMRV and the aforementioned RNASEL polymorphism was not found 2 the idea that defects in innate immunity might be linked to XMRV infection prompted others to look for the virus in patients with CFS 3 . Remarkably PCR assays identified XMRV DNA in peripheral blood samples from 68 of 101 CFS patients and 8 of 218 healthy controls.