tailieunhanh - Báo cáo y học: " siRNA and shRNA screens advance key understanding of host factors required for HIV-1 replication"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Retrovirology cung cấp cho các bạn kiến thức về ngành y đề tài: " siRNA and shRNA screens advance key understanding of host factors required for HIV-1 replication. | Retrovirology BioMed Central Commentary siRNA and shRNA screens advance key understanding of host factors required for HIV-1 replication Kin-Hang Kok Ting Lei and Dong-Yan Jin Open Access Address Department of Biochemistry the University of Hong Kong 21 Sassoon Road Pokfulam Hong Kong PR China Email Kin-Hang Kok - khkok@ Ting Lei - leiting@ Dong-Yan Jin - dyjin@ Corresponding author Published 27 August 2009 Received 19 July 2009 Retrovirology 2009 6 78 doi 1742-4690-6-78 Accepted 27 August 2009 This article is available from http content 6 1 78 2009 Kok et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract A recent RNAi screen used a genome-wide shRNA library to search for cellular factors required for HIV-1 replication. This work complements three other siRNA-based screening studies and potentially opens the door to the discovery of factors that are important for HIV-1 replication in physiological host cells such as T lymphocytes. shRNA screens can be further improved and they could promise to unravel new pathways and new facets of virus-cell interactions. Commentary The advent of RNAi-based whole-genome screens in mammalian cells provides a new impetus to the search of host cell factors needed for HIV replication 1 2 . Three screens that used siRNA pools to identify cellular proteins important in HIV-1 replication were reported in 2008 and a meta-analysis of these studies has been published recently 3-6 . One shortcoming to these reported screens is the use of HeLa or HEK293T cells that are not physiological substrates for infection by HIV-1. In addition the use of a pseudotyped virus or a mutated strain of HIV-1 also limits the interpretability of some

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