tailieunhanh - Báo cáo y học: "Identification of an endogenous retroviral envelope gene with fusogenic activity and placenta-specific expression in the rabbit: a new "syncytin" in a third order of mammals"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Retrovirology cung cấp cho các bạn kiến thức về ngành y đề tài: "Identification of an endogenous retroviral envelope gene with fusogenic activity and placenta-specific expression in the rabbit: a new "syncytin" in a third order of mammals. | Retrovirology BioMed Central Research Identification of an endogenous retroviral envelope gene with fusogenic activity and placenta-specific expression in the rabbit a new syncytin in a third order of mammals Odile Heidmannt Cécile Vernochett Anne Dupressoir and Thierry Heidmann Open Access Address Unité des Rétrovirus Endogènes et Elements Rétroides des Eucaryotes Supérieurs CNRS UMR 8122 Institut Gustave Roussy 39 rue Camille Desmoulins F-94805 Villejuif and Université Paris-Sud Orsay F-91405 France Email Odile Heidmann - oheidmann@ Cécile Vernochet - vernochet@ Anne Dupressoir - dupresso@ Thierry Heidmann - heidmann@ Corresponding author tEqual contributors Published 27 November 2009 Received 22 October 2009 Accepted 27 November 2009 Retrovirology 2009 6 107 doi 1742-4690-6-107 This article is available from http content 6 1 107 2009 Heidmann et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Syncytins are envelope genes of retroviral origin that have been co-opted by the host to mediate a specialized function in placentation. Two of these genes have already been identified in primates as well as two distinct non orthologous genes in rodents. Results Here we identified within the rabbit Oryctolagus cuniculus-which belongs to the lagomorpha order- an envelope env gene of retroviral origin with the characteristic features of a bona fide syncytin that we named syncytin-Oryl. An in silico search for full-length envgenes with an uninterrupted open reading frame within the rabbit genome first identified two candidate genes that were tested for their specific expression in the placenta by quantitative RT-PCR of RNA isolated from a large set of tissues.