tailieunhanh - Báo cáo y học: " Reduced levels of reactive oxygen species correlate with inhibition of apoptosis, rise in thioredoxin expression and increased bovine leukemia virus proviral loads"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Retrovirology cung cấp cho các bạn kiến thức về ngành y đề tài: Reduced levels of reactive oxygen species correlate with inhibition of apoptosis, rise in thioredoxin expression and increased bovine leukemia virus proviral loads. | Retrovirology BioMed Central Research Reduced levels of reactive oxygen species correlate with inhibition of apoptosis rise in thioredoxin expression and increased bovine leukemia virus proviral loads Amel Baya Bouzar1 3 Mathieu Boxus1 Arnaud Florins1 Carole Francois1 Michal Reichert2 and Luc Willems 1 3 Address 1Université de Liège ULg Gembloux Agro-Bio Tech Molecular and Cellular Biology Gembloux Belgium 2National Veterinary Research Institute Pulawy Poland and interdisciplinary Cluster for Applied Genoproteomics GIGA University of Liège ULg Liège Belgium Email Amel Baya Bouzar - Mathieu Boxus - Arnaud Florins - Carole Francois - Michal Reichert - reichert@ Luc Willems - Corresponding author Open Access Published 10 November 2009 Received 28 May 2009 Accepted 10 November 2009 Retrovirology 2009 6 102 doi 1742-4690-6-102 This article is available from http content 6 1 102 2009 Bouzar et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Bovine Leukemia virus BLV is a deltaretrovirus that induces lymphoproliferation and leukemia in ruminants. In ex vivo cultures of B lymphocytes isolated from BLV-infected sheep show that spontaneous apoptosis is reduced. Here we investigated the involvement of reactive oxygen species ROS in this process. Results We demonstrate that i the levels of ROS and a major product of oxidative stress 8-OHdG are reduced while the thioredoxin antioxidant protein is highly expressed in BLV-infected B lymphocytes ii induction of ROS by valproate VPA is pro-apoptotic iii inversely the scavenging of ROS with N-acetylcysteine inhibits .