tailieunhanh - Báo cáo y học: " Synergistic effect of human CycT1 and CRM1 on HIV-1 propagation in rat T cells and macrophages"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Retrovirology Research cung cấp cho các bạn kiến thức về ngành y đề tài:"Synergistic effect of human CycT1 and CRM1 on HIV-1 propagation in rat T cells and macrophages. | Retrovirology BioMed Central Research Synergistic effect of human CycTl and CRM1 on HIV-1 propagation in rat T cells and macrophages Hiroyuki Okada 1 Xianfeng Zhang1 Ismael Ben Fofana1 2 Mika Nagai1 Hajime Suzuki1 Takashi Ohashi1 and Hisatoshi Shida 1 Open Access Address 1Institute for Genetic Medicine Hokkaido University Kita-ku Sapporo 060-0815 Japan and 2Microbiology Division New England Primate Research Center Harvard Medical School One Pine Hill Drive Southborough Maryland 01772 USA Email Hiroyuki Okada - hiro1230@ Xianfeng Zhang - zhangxf@ Ismael Ben Fofana - Ismael_Fofana@ Mika Nagai - purefood@ Hajime Suzuki - hjsuzuki@ Takashi Ohashi - ohashi-t@ Hisatoshi Shida - hshida@ Corresponding author Published 12 May 2009 Received II September 2008 Retrovirology 2009 6 43 doi 1742-4690-6-43 Accepted 12 May 2009 This article is available from http content 6 1 43 2009 Okada et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background In vivo studies of HIV-1 pathogenesis and testing of antiviral strategies have been hampered by the lack of an immunocompetent small animal model that is highly susceptible to HIV- I infection. Although transgenic rats that express the HIV-1 receptor complex hCD4 and hCCR5 are susceptible to infection HIV-1 replicates very poorly in these animals. To demonstrate the molecular basis for developing a better rat model for HIV-1 infection we evaluated the effect of human CyclinT1 hCycT1 and CRM1 hCRM1 on Gag p24 production in rat T cells and macrophages using both established cell lines and primary cells prepared from hCycT1 hCRM1 transgenic .