tailieunhanh - Retrovirology Research BioMed Central Open Access A role for CD81 on the late steps of HIV-1

Retrovirology Research BioMed Central Open Access A role for CD81 on the late steps of HIV-1 replication in a chronically infected T cell line Boyan Grigorov1, Valérie Attuil-Audenis1, Fabien Perugi2, Martine Nedelec2, Sarah Watson1, Claudine Pique2, Jean-Luc Darlix1, Hélène Conjeaud2 and Delphine Muriaux*1 Address: 1LaboRetro, Unité de virologie humaine INSERM U758, Ecole Normale Supérieure de Lyon, IFR128, 46 allée d'Italie, 69364 Lyon, France and 2Institut Cochin, Département de Biologie Cellulaire, CNRS 8104, INSERM 567, Paris V, 22 rue Méchain, 75014 Paris, France Email: Boyan Grigorov - ; Valérie Attuil-Audenis - ; Fabien Perugi - ; Martine Nedelec - ; Sarah Watson - ; Claudine Pique -. | Retrovirology BioMed Central Research A role for CD8I on the late steps of HIV-1 replication in a chronically infected T cell line Boyan Grigorov1 Valérie Attuil-Audenis1 Fabien Perugi2 Martine Nedelec2 Sarah Watson1 Claudine Pique2 Jean-Luc Darlix1 Hélène Conjeaud2 and Delphine Muriaux 1 Open Access Address 1LaboRetro Unité de virologie humaine INSERM U758 Ecole Normale Supérieure de Lyon IFR128 46 allée d Italie 69364 Lyon France and 2Institut Cochin Département de Biologie Cellulaire CNRS 8104 INSERM 567 Paris V 22 rue Méchain 75014 Paris France Email Boyan Grigorov - Valérie Attuil-Audenis - Fabien Perugi - Martine Nedelec - Sarah Watson - Claudine Pique - jldarlix@ Jean- Luc Hélène Conjeaud - Delphine Muriaux - dmuriaux@ Corresponding author Published II March 2009 Retrovirology 2009 6 28 doi 1742-4690-6-28 Received 3I October 2008 Accepted II March 2009 This article is available from http content 6 I 28 2009 Grigorov et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ Background HIV-I uses cellular co-factors for virion formation and release. The virus is able to incorporate into the viral particles host cellular proteins such as tetraspanins which could serve to facilitate HIV-I egress. Here we investigated the implication of several tetraspanins on HIV-I formation and release in chronically infected T-lymphoblastic cells a model that permits the study of the late steps of HIV-I replication. .

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