tailieunhanh - Retrovirology Research BioMed Central Open Access Modulation of HIV-1 infectivity and cyclophilin

Retrovirology Research BioMed Central Open Access Modulation of HIV-1 infectivity and cyclophilin A-dependence by Gag sequence and target cell type Saori Matsuoka1,2, Elisabeth Dam1,3, Denise Lecossier1,2, François Clavel1,2 and Allan J Hance*1,2 Address: 1INSERM U941, Paris 75010, France, 2Institut Universitaire d'Hématologie, Université Paris Diderot, Paris 75010, France and 3BioAlliancePharma, Paris 75015, France Email: Saori Matsuoka - ; Elisabeth Dam - ; Denise Lecossier - ; François Clavel - ; Allan J Hance* - * Corresponding author Published: 2 March 2009 Retrovirology 2009, 6:21 doi: Received: 9 January 2009 Accepted: 2 March 2009 This article is available from: © 2009 Matsuoka et al; licensee BioMed Central Ltd. This. | BioMed Central Retrovirology Research Modulation of HIV-I infectivity and cyclophilin A-dependence by Gag sequence and target cell type Saori Matsuoka1 2 Elisabeth Dam1 3 Denise Lecossier1 2 Francois Clavel1 2 and Allan J Hance 1 2 Open Access Address 1INSERM U941 Paris 75010 France 2Institut Universitaire d Hématologie Université Paris Diderot Paris 75010 France and 3BioAlliancePharma Paris 75015 France Email Saori Matsuoka - Elisabeth Dam - Denise Lecossier - Francois Clavel - Allan J Hance - Corresponding author Published 2 March 2009 Received 9 January 2009 Accepted 2 March 2009 Retrovirology 2009 6 21 doi l 742-4690-6-21 This article is available from http content 6 1 21 2009 Matsuoka et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background HIV-1 Gag proteins are essential for virion assembly and viral replication in newly infected cells. Gag proteins are also strong determinants of viral infectivity immune escape mutations in the Gag capsid CA protein can markedly reduce viral fitness and interactions of CA with host proteins such as cyclophilin A CypA and TRIM5a can have important effects on viral infectivity. Little information however is available concerning the extent that different primary Gag proteins affect HIV-1 replication in different cell types or the impact on viral replication of differences in the expression by target cells of proteins that interact with CA. To address these questions we compared the infectivity of recombinant HIV-1 viruses expressing Gag-protease sequences from primary isolates in different target cells in

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