tailieunhanh - Báo cáo hóa học: " Pyrosequencing of 16S rRNA gene amplicons to study the microbiota in the gastrointestinal tract of carp (Cyprinus carpio L.)"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí hóa hoc quốc tế đề tài : Pyrosequencing of 16S rRNA gene amplicons to study the microbiota in the gastrointestinal tract of carp (Cyprinus carpio L.) | o AMB Express a SpringerOpen Journal van Kessel et al. AMB Express 2011 1 41 http content 1 1 41 ORIGINAL Open Access Pyrosequencing of 16S rRNA gene amplicons to study the microbiota in the gastrointestinal tract of carp Cyprinus carpio L. K laartío A I I I 2ti kfoccol1 2 Rac R rii ifi I h3 4 kAmolia Mox olinn5 Í Iir kì2í2il p lÓA int5 Imric A plfman5 Plil 2 Mddrtje AHJ vdn Kessel Dds E DutilH Kornelid Neveling Michdel P KVVUlt Joris A veluildn Gelt Flik Mike SM Jetten 1 Peter HM Kldren2 dnd Huub JM Op den Cdmp1 Abstract The microbes in the gdstrointestindl GI tract dre of high importance for the hedlth of the host. In this study Roche 454 pyrosequencing wds dpplied to d pooled set of different 16S rRNA gene dmplicons obtdined from GI content of common cdrp Cyprinus carpio to mdke dn inventory of the diversity of the microbiotd in the GI tract. Compdred to other studies our culture-independent investigdtion revedls dn impressive diversity of the microbidl flord of the cdrp GI tract. The mdjor group of obtdined sequences belonged to the phylum Fusobacteria. Bacteroidetes Planctomycetes dnd Gammaproteobacteria were other well represented groups of micro-orgdnisms. Verrucomicrobiae Clostridia dnd Bacilli the ldtter two belonging to the phylum Firmicutes hdd fewer representdtives dmong the dndlyzed sequences. Mdny of these bdcterid might be of high physiologicdl relevdnce for cdrp ds these groups hdve been implicdted in vitdmin production nitrogen cycling dnd cellulose fermentdtion. Keywords intestindl tract biodiversity cdrp dqudculture pyrosequencing 16S rRNA Introduction The intestine is a multifunctional organ system involved in the digestion and absorption of food electrolyte balance endocrine regulation of food metabolism and immunity against pathogens Ringo et al. 2003 . The gastrointestinal GI tract is inhabited by many different micro-organisms. As in mammals this dynamic population of micro-organisms is of key importance for the health of