tailieunhanh - Báo cáo khoa học: Dermaseptin DA4, although closely related to dermaseptin B2, presents chemotactic and Gram-negative selective bactericidal activities

Antimicrobial peptides participate in innate host defense by directly elimi-nating pathogens as a result of their ability to damage the microbial mem-brane and by providing danger signals that will recruit innate immune cells to the site of infection. Dermaseptin DA4 (DRS-DA4), a new antimicrobial peptide of the dermaseptin superfamily, was identified based on its chemo-tactic properties, contrasting with the currently used microbicidal proper-ties assessment. | Dermaseptin DA4 although closely related to dermaseptin B2 presents chemotactic and Gram-negative selective bactericidal activities Constance Auvynet1 2 Pierre Joanne2 f Julie Bourdais3 Pierre Nicolas2 f Claire Lacombe2 4 Ị and Yvonne Rosenstein1 1 Departamento de Medicina Molecular y Bioprocesos Institute de Biotecnologia Universidad NacionalAutonoma de Mexico ColChamilpa Cuernavaca Morelos Mexico 2 FRE 2852 Peptidome de la peau des amphibiens CNRS Universite Paris-Pierre et Marie Curie Paris France 3 Independent scholar Cuernavaca Morelos Mexico 4 UFR Sciences et Technologie Universite Paris 12-Valde Marne Creteil France Keywords antimicrobialpeptide chemotaxis dermaseptin frog skin peptide-membrane interactions Correspondence Y. Rosenstein Departamento de Medicina Molecular y Bioprocesos Instituto de Biotecnologia Universidad Nacional Autonoma de Mexico Avenida Universidad 2001 ColChamilpa Cuernavaca Morelos 62270 Mexico Fax 52 73172388 Tel 52 5 55 66 22 76 63 E-mail yvonne@ C. Lacombe Laboratoire des Biomolécules Universite Pierre et Marie Curie-CNRS-ENS 4 Place Jussieu 75252 Paris cedex 05 France Fax 33 1 44 27 55 64 Tel 33 1 44 27 51 59 E-mail Present addresses INSERM UMR-S 945 Immunite et Infection Universite Pierre et Marie Curie Paris France fBiogenese des Signaux Peptidiques BIOSIPE ER3-UPMC Universite Pierre et Marie Curie Paris France Antimicrobial peptides participate in innate host defense by directly eliminating pathogens as a result of their ability to damage the microbial membrane and by providing danger signals that will recruit innate immune cells to the site of infection. Dermaseptin DA4 DRS-DA4 a new antimicrobial peptide of the dermaseptin superfamily was identified based on its chemotactic properties contrasting with the currently used microbicidal properties assessment. The peptide was isolated and purified by size exclusion HPLC and RP-HPLC from the skin of the Mexican frog Pachymedu-sa dacnicolor. MS and .