tailieunhanh - Báo cáo hóa học: " Effects of internal low-dose irradiation from 131 I on gene expression in normal tissues in Balb/c mice"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí hóa hoc quốc tế đề tài : Effects of internal low-dose irradiation from 131 I on gene expression in normal tissues in Balb/c mice | Schuler et al. EJNMMI Research 2011 1 29 http content 1 1 29 9 EJNMMI Research a SpringerOpen Journal ORIGINAL RESEARCH Open Access Effects of internal low-dose irradiation from 131I on gene expression in normal tissues in Balb c mice 1 T I -7 n 2 1 2 1 Emil Schuler Toshima Z Parris Nils Rudqvist Khalil Helou and Eva Forssell-Aronsson Abstract Background The aim of this study was to investigate the global gene expression response of normal tissues following internal low absorbed dose irradiation of 131I. Methods Balb c mice were intravenously injected with 13 to 260 kBq of 131I and euthanized 24 h after injection. Kidneys liver lungs and spleen were surgically removed. The absorbed dose to the tissues was to mGy. Total RNA was extracted and Illumina MouseRef-8 Whole-Genome Expression BeadChips Illumina Inc. San Diego California USA were used to compare the gene expression of the irradiated tissues to that of non-irradiated controls. The Benjamini-Hochberg method was used to determine differentially expressed transcripts and control for false discovery rate. Only transcripts with a modulation of or higher either positively or negatively regulated were included in the analysis. Results The number of transcripts affected ranged from 260 in the kidney cortex to 857 in the lungs. The majority of the affected transcripts were specific for the different absorbed doses delivered and few transcripts were shared between the different tissues investigated. The response of the transcripts affected at all dose levels was generally found to be independent of dose and only a few transcripts showed increasing or decreasing regulation with increasing absorbed dose. Few biological processes were affected at all absorbed dose levels studied or in all tissues studied. The types of biological processes affected were clearly tissue-dependent. Immune response was the only biological process affected in all tissues and processes affected in more than .