tailieunhanh - Báo cáo y học: "Rho iso-alpha acids from hops inhibit the GSK-3/NF-κB pathway and reduce inflammatory markers associated with bone and cartilage degradation"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Rho iso-alpha acids from hops inhibit the GSK-3/NF-κB pathway and reduce inflammatory markers associated with bone and cartilage degradation. | Journal of Inflammation BioMed Central Research Rho iso-alpha acids from hops inhibit the GSK-3 NF-kB pathway and reduce inflammatory markers associated with bone and cartilage degradation Veera Reddy Konda Anuradha Desai Gary Darland Jeffrey S Bland and Matthew L Tripp Open Access Address MetaProteomics Nutrigenomics Research Center a subsidiary of Metagenics Inc 9770 44th Avenue . Gig Harbor WA 98332 USA Email Veera Reddy Konda - vrkonda@ Anuradha Desai - anudesai@ Gary Darland - garydarland@ Jeffrey S Bland - jeffbland@ Matthew LTripp - matthewtripp@ Corresponding author Published 27 August 2009 Received 28 January 2009 Accepted 27 August 2009 Journal of Inflammation 2009 6 26 doi 1476-9255-6-26 This article is available from http content 6 1 26 2009 Konda et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Rho iso-alpha acids RIAA from hops have been shown to have anti-inflammatory properties. To understand the mechanisms we evaluated the effect of RIAA in cell signaling pathways and inflammatory markers using various in vitro models. We also investigated their therapeutic effect in mice with collagen-induced arthritis. Methods The LPS-stimulated RAW macrophages were used to evaluate the effect of RIAA on the NF-kB and MAPK signaling pathways phosphorylation of ERKI 2 p38 and JNK was assessed by western blotting and NF-kB binding by electrophoretic mobility shift assays. Effect on the NF-kB activity was evaluated by the luciferase reporter assays in LPS-stimulated RAW cells. GSK-3a p kinase activity was measured in cell-free assays. The inhibitory effect of RIAA on inflammatory